PHYSIOLOGICAL PROCESSES IN SOYBEAN INHIBITED BY GASEOUS POLLUTANTS BUT NOT BY ACID RAIN

A high-affinity Ca2+ pump, ECA1, from the endoplasmic reticulum is inhibited by cyclopiazonic acid but not by thapsigargin.

To identify and characterize individual Ca2+ pumps, we have expressed an Arabidopsis ECA1 gene encoding an endoplasmic reticulum-type Ca2+-ATPase homolog in the yeast (Saccharomyces cerevisiae) mutant K616. The mutant (pmc1pmr1cnb1) lacks a Golgi and a vacuolar membrane Ca2+ pump and grows very poorly on Ca2+-depleted medium. Membranes isolated from the mutant showed high H+/Ca2+-antiport but n...

A High-Affinity Ca Pump, ECA1, from the Endoplasmic Reticulum Is Inhibited by Cyclopiazonic Acid but Not by Thapsigargin

To identify and characterize individual Ca pumps, we have expressed an Arabidopsis ECA1 gene encoding an endoplasmic reticulum-type Ca-ATPase homolog in the yeast (Saccharomyces cerevisiae) mutant K616. The mutant (pmc1pmr1cnb1) lacks a Golgi and a vacuolar membrane Ca pump and grows very poorly on Ca-depleted medium. Membranes isolated from the mutant showed high H/Ca-antiport but no Ca-pump a...

Absorption of Gaseous Air Pollutants By a Standardized Plant Canopy

Adsorption of Gaseous Pollutants

Magnesium Chelatase of Hordeum vulgare L. Is Not Activated by Light but Inhibited by Pheophorbide

The enzyme activity of magnesium chelatase was determined in intact etioplasts of barley ( Hordeum vulgare L.) seedlings. Irradiation of isolated plastids with white light for 15 min does not lead to any activation of the enzyme but to a decrease in activity, especially in etioplasts. The enzyme was inhibited by chlorophyllide and zinc pheophorbide only to a certain extent. Strong inhibition wa...